Luke Slaughter of Tombstone was a shortlived CBS old-time radio series that aired between Frontier Gentleman and Have Gun Will Travel, from February 23 to June 15, 1958. The show starred Sam Buffington as Luke Slaughter, a Civil War cavalryman turned Arizona cattleman, and Junius Matthews as his sidekick, Wichita. Directed by acclaimed radio producer William N. Robson, the show featured well-written and well-acted scripts that presented Luke Slaughter as a tough, no-nonsense cowboy who faced various challenges as he and his partner tried to run their cattle business.

Despite its high quality, Luke Slaughter of Tombstone was canceled after only 16 episodes due to the growing popularity of television. Buffington's portrayal of the hard-boiled cowboy has often been compared to William Conrad's Matt Dillon on Gunsmoke.